Journal: The American journal of pathology
Article Title: Tertiary Lymphoid Structures in Rheumatoid Arthritis: NF-κB-Inducing Kinase-Positive Endothelial Cells as Central Players.
doi: 10.1016/j.ajpath.2015.03.012
Figure Lengend Snippet: Figure 4 Follicular dendritic cells (FDCs) and platelet-derivedgrowth factorreceptorb (PDGFRb)þ
Article Snippet: Immunofluorescence Staining Frozen ST biopsy samples or lymph node tissues were cut in 5-mm sections, fixed with acetone, and incubated with the following: goat anti-CD31 (sc-1505; Santa Cruz Biotechnology) and anti-CD34 (sc-7045; SantaCruzBiotechnology); mouse monoclonal anti-NIK (sc-8417; Santa Cruz Biotechnology), FDC-M1 (14-9968-80; eBioscience), rabbit polyclonal primary antibodies against PDGFRb (sc-432; Santa Cruz Biotechnology), rabbit anti-CD3 (1501-1; Epitomics, Burlingame, CA), rabbit anti-CD20 (1632-1; Epitomics), rat anti-RORgamma (t) (14-6981; eBioscience, San Diego, CA), rat anti-PNAd (clone MECA-79; BioLegend, San Diego, CA), and goat anti-CXCL13 (AF801; R&D Systems, Minneapolis, MN).
Techniques: